A Standard Curve Developed From The Cytoplasmic Protein...

Figure 1 shows a standard curve developed from the cytoplasmic protein standards of lung cancer cells. A gradual increase in corrected absorbance can be notes, which more noticeably increases after 125 ÃŽ ¼g/mL. After this point the values nearly double. The absorbance readings for the samples were recorded along with the standard concentrations [500 ÃŽ ¼g/mL, 250 ÃŽ ¼g/mL, 125 ÃŽ ¼g/mL, 62.5 ÃŽ ¼g/mL, and 0 ÃŽ ¼g/mL (PBS only) †¨The corrected absorbance for the protein standards were found by subtracting the average absorbance of the PBS blanks (0 ÃŽ ¼g of protein) from all other averages. †¨Excel was then used to create a standard curve for the above protein standards, where protein concentration was plotted on the X-axis and corrected absorbances on the y-axis. The linear trend line was used to determine the protein concentration of the diluted sample according to the average corrected absorbance values. The dilution factor was accounted for by multiplying the calculated concentrations of all protein samples by 20. The volume (in ÃŽ ¼L) needed to load an equal amount of the volume (in ÃŽ ¼g) of the second protein sample was calculated, followed by the calculation of the RIPA volume needed to fill the protein sample to 10 ÃŽ ¼L. Four times the volume was then calculated for each volume and these values were used for the SDS-PAGE procedure the following week. The sample with the lowest concentration was used to calculate the amount of protein (in ÃŽ ¼g) used in the 10 ÃŽ ¼L solution, indicating the maximum

Personal Narrative Short Story - 1538 Words

I open my eyes at the sound of someone’s voice. I know that voice-- it’s†¦ â€Å"Ugh! Sylvia! Did you seriously think you were going to win that fistfight against Tammy Williams?!† my best friend Eileen says standing over me as I lay down on the concrete of the school basketball courts. Everything hurts as I try to get up from being unconscious for god knows how long. As I get up, and Eileen hands me my backpack. She told me Tammy jumped me after me â€Å"talking trash† about her, which I didn’t do. So you probably figured I’m Sylvia. Seventh grade, probably dumbest person in the school. Not kidding, take a look at my report card from last semester and you’ll be shocked about how many F’s there are. I also have a really active imagination, so I just†¦show more content†¦I get confused but then notice that not just my skin is getting lighter, but everything else around me is. Everything suddenly looks white, then I wake up from lying on the floor, again. My head still hurting from when Tammy Williams probabl y round-house kicked it. Annoyed from having to get up, I decide to stay there staring at the ceiling of wherever I am, not wanting to get up. But suddenly, this guy is standing above me. He looks familiar, but I can’t tell where I’ve seen him from. â€Å"Do you need help?† he asks. Embarrassed, I quickly get up, but I got up too fast so then I fall back down to where I was, making it even more embarrassing. â€Å"Sylvia, what were you doing on the floor of the restaurant?† That was when I knew I knew this man. His name was Nate, and he was from one of the stories I imagined in my head. I feel something in my hand, so I look, but it’s just that glass thing that was hanging on the door. I shove it in my back pocket trying to figure out what’s going on. I look around, and it’s exactly how I imagined it. I look down to see if anything else has changed like it was supposed to, but it didn’t. I’m still dirty from when I was laying on the ground after Tammy beat me up, and I still have the same curly hair that I absolutely hate. â€Å"Uh, yeah I’m fine†¦ Nate†¦Ã¢â‚¬  I say uncomfortably. I guess he picked up on that, so then he looked down at himself. â€Å"What? Is there something onShow MoreRelatedPersonal Narrative Short Story1153 Words   |  5 PagesI still remember that fateful day, the day I was Stolen. It started out as a normal day at school. I’ll take you through the whole story. There is one thing you should try to figure out, who kidnapped me. â€Å"Joey, Wait up!† I yelled out. Why was he walking so fast, and not responding? It’s almost like he’s ignoring me. I started to jog, but he was already gone. I turned the other way to walk home. It was a long walk, for I lived in the country part of town. I noticed leaves crunching behind me, andRead MorePersonal Narrative Short Story1191 Words   |  5 PagesIt was a warm summer day with good air, most kids were out riding their bikes, scooters, etc. or running around, playing tag, yet I was huddled inside, reading a good book. The sun was shining bright, yet the clouds were blocking it, acting like the filter in a juice machine, separating the good stuff from the bad stuff. The clouds let the light come in just enough so it can light up everything, but not enough to make you sweat when not doing draining activities. This made it the perfect weatherRead MorePersonal Narrative : A Short Story1301 Words   |  6 Pages â€Å"Aww is little Airi hurt?† he says with a sarcastic tone. His let’s out his evil laugh and picks me up. â€Å"L-leave me alone.. DON’T TOUCH ME!† I scream out. â€Å"Okay† he says as he immediately drops me on the floor and drags me through the snow by my short brown hair. My legs are numb so I can’t feel my legs being cut up by the wooden porch. He lets go of my hair in the middle of the living room. This is the first time he’s ever put his hands on me and I don’t like it. My mother comes back and takesRead MorePersonal Narrative : A Short Story1598 Words   |  7 PagesShadows The leaves blowing, the wind was whistling. The moon shined brighter than the sun did that morning. I looked like no other normal teenager, But I was wearing all black. Black leggings, Black jacket, Black shoes, black shirt. My hair looked like caramel, my eyes looked like a yellowish brown that shined in the moonlight. My name was nothing important to people. People say â€Å"Angelys† is a nice name but i think differently. Nothing seemed to happen, I was coming home from a friend’s house.Read MorePersonal Narrative Short Story1415 Words   |  6 Pagesme have alcohol, and would only get me some water. At the end of the day, I had seen many people and the many occupations they all had. I saw many people from different jobs including, fishermen, merchants, importers, and bankers. I heard many stories of people’s families. As night approached, I walked out of the coffeehouse in search of somewhere to sleep. I did not want to go back to my own house because I did not want to catch yellow fever myself. So I decided to leave town, and walk all theRead MorePersonal Narrative Short Story1598 Words   |  7 Pages â€Å"Noooooooooo, I don’t want to!† I yelled. â€Å"I don’t care Emma, I’m going to be gone all night,† my mom said. â€Å"I am not babysitting Ally. I have track tonight.† â€Å"I bet you can make it up another day. You are babysitting your little sister tonight no matter what.† â€Å"No I’m not!† â€Å"I don’t like your attitude Emma! If you don’t babysit your little sister tonight, you can’t do track anymore!† â€Å"I hate you! I hate you! I hate you!† Then I ran out of the house slamming the door behind me. My momRead MorePersonal Narrative Story Short Story1619 Words   |  7 PagesSarah and Roger with their twins, Jules and Kendra. All of us cousins were very close in age. Stewart was the eldest, at age fourteen he was four years older than the youngest but he still hung out with us on the beach after dark. He had the best stories out of all of us. Andrew and I were the next oldest, six months apart in age in fact. The twins and Eric were the youngest. I remember the twins had just celebrated their 12th birthday that earlier that summer, while Eric’s birthday wasn’t untilRead MorePersonal Narrative Short Story1787 Words   |  8 Pagescaught by one of the S.W.A.T. officers. I remember being in a helicopter and being brought to a tower. I was led to a room with a gun pointed at my back, I wanted to run and get my life over with but I knew that my job was more important than my personal wants. I saw a man his face was handsome but his eyes held the death of hundreds. I knew that my time here was not going to be leisurely. This man began asking me questions such as what I was doing here and why I was running from the S.W.A.T. I ofRead MorePersonal Narrative Essay : A Short Story936 Words   |  4 Pageswhen I open the door of my grandma’s house my grandma asks â€Å"Are you ok?† â€Å"Your face is all red† â€Å"I’m fine,† I say. Then I run to my room trying to hide my tears. Whenever I feel bad I would always read a bedtime story book but not the ones with happy endings. So I grab are small story called anger. There was once a man who kept all his sadness and anger in him waiting to explode and finally one day he couldn’t take it anymore and all that anger exploded the end. After I read it I realized it remindedRead MoreAlice Munros an Ounce of Cure1548 Words   |  7 Pagesstudies make the link between literary techniques such as narrative and storytelling and several other disciplines. Thus, one may find the theories of narrative and storytelling extending up to several major disciplines, such as philosophy, psychology, and literary criticism. In the view of different experts in literary analyses, there is a close connection between narrative or storytelling and the definition of the nature of self and personal identity. The former has been understood as influential

Fitness Personal Life and Healthy Active Lifestyle free essay sample

You can consider yourself to have a healthy lifestyle if you have good physical, mental, social, and spiritual health. Good physical health is a result of all your bodys important functions working well together. A person in good physical health can more easily to fight off disease, recover from illness, and perform daily routines without feeling tired. Good mental health is a result of positive feelings about you and about others. Your mental health can influence your decisions. A person in good mental health can more easily to deal with stress, cope with change and maintain a positive outlook on life. Good social health is a result of your interactions with others and of coping well with social situations. Social health can be built through friendships, participation in community groups, and volunteer work. A person in good social health can more easily to feel close and connected to other people, understand his or her own self-worth and cope with lifes ups and downs. Good spiritual health gives peace of mind. Spirituality can be gained and expressed in the way you play a musical instrument, dance, work with art materials, or through religion. It can also be apparent through reaching out to help others in ways that restore their dignity and self-worth. Good spiritual health can give your life a purpose to provide you with stress relief and help you develop support systems. Focusing on each of these dimensions, and continually striving to improve in each area, is one of the keys to a long and happy life. 2. What is BMI? What is your BMI ? Body Mass Index (BMI) is a number calculated from a persons weight and height. BMI is a fairly reliable indicator of body fatness for most people. BMI does not measure body fat directly, but research has shown that BMI correlates to direct measures of body fat, such as underwater weighing and dual energy x-ray absorptiometry (DXA). 1, 2  BMI can be considered an alternative for direct measures of body fat. Additionally, BMI is an inexpensive and easy-to-perform method of screening for weight categories that may lead to health problems. Calculating BMI BMI=__________________ 3. What is long-term fitness goal that you aspire to? 4. What are different ways to live a healthy active lifestyle ? Nutrition 1. Why should Nutrition be important for teenager? 2. What are 4 food groups and why they are important? 3. Create 3-days meal plan that incorporate all the food groups and contributes to a healthy active lifestyle. Your meal plan should include specific serving size. | B/L/D/S| Serving size| | DAY 1| | | | DAY 2| | | | DAY 3| | | | Sexuality 1. What are the 10 component that you want in a healthy relationship? 10 Component of Healthy Relationship * Feel happy and relaxed * Spend time together and time apart * Demonstrate trust * Are confident Have positive self-esteem * Act with mutual respect * Feel appreciated * Communicate openly and honesty * Attempt to understand one another * Listen to each other 2. What is Abstinence? Why is Abstinence the best method to protect your sexual health? Abstinence from sexual activity means refraining from any form of sexual activity that could result in pregnancy or the all form of intercourse. Abstinence is the best method to protect s exual health 3. What are the 2 common STI’s? Indicate how they are transmitted, sign and symptoms, treatment and prevention. Name of STI’s| Transmitted| Sign and Symptoms | Treatment and Prevention| HPV(Human Papilloma Virus)| * Unprotected sex * Direct contact with sores/kissing * Mother to infant before birth| * Stage 1= small red bumps, liquid in genital area (chancres) * Stage 2= rash, flu-like symptoms, hair loss, genital growth| * Antibiotics, but only if it is caught easy * If not treated early, medication cannot repair damage already done| Trichomonas | * Unprotected sex or sexual contact| * Saginal discharge and odour * Pain or itching (during urinating) * Spotty bleeding frequent urination | * Antibiotics for affected person and his or her partner| 4. What are 2 common STI’s of contraceptive and indicate how it works, its effectiveness, advantages and disadvantage? Name of Contraceptive| How it work| Effectiveness| Advantages| Disadvantages| Male Condom | * Physical barrier acts to prevent direct genital contact and the exchange of genital fluids| * The condom is 98% effective when used perfectly * With typical use, it is 85% effective| * Available without a prescription * Latex condoms protect against sexually transmitted infection * May help to avoid premature ejaculation | * Must be | | | | | | Conflict Resolution 1. What are 4 type of conflict that may affect teenagers and how can they be deal with effective? 4 type of conflict that may affect teenagers a. Internal Conflicts b. Interpersonal Conflicts c. Intra-Group conflicts d. Inter-Group Conflicts 2. What skills can help teenagers deal with conflicts? Which of those attribute do you oppose? a. Empathy b. Patience and to Lawrence c. Clear and direct message d. Creative thinking e. Critical thinking f. Assertiveness Body Image and self-esteem 1. What is body image? What is self-esteem? Body image is an mental picture of your own body. Body image includes how you feel about your body, want you believe about your body, how you see yourself and how you think you look to others. Self-esteem describes how valued or worthy a person feels. People with high-self-esteem make good choices. People with low-self-esteem may feel that they are unworthy of the opportunities life has to offer. 2. What are 3 factors that influence body image? The 3 factors that influence body image are impact of media on body image, family influence on body image, and friend’s effect on body image. 3. What is the self-point theory? Self-Point Theory says each of us has a weight (or â€Å"self-point†) that our body â€Å"programmed† to maintaingt; if we drop below this self-point the theory says our body automatically reacts (possible to protect itself) and we are unable to keep weight off. 4. How would you rate your body image on a scale of 1-100 and why? Exercise Activity 1. What type of exercise do you most enjoy and why? 2. Indicate where your exercise originated and the basic concepts of the exercise, equipment needed and space required.

Younger Dryas in the UK Essay Example

Younger Dryas in the UK Essay The Younger Dryas refers to the final phase of cold, glacial conditions preceding the abrupt climatic warming at the beginning of the Holocene. The existence of the Younger Dryas in Europe has been known for most of this century, although recent research suggests that the Younger Dryas cooling may have been global. Estimates of the timing of the event have also improved in recent years, showing that both the onset and termination of the Younger Dryas were abrupt, occurring within decades. The Younger Dryas has been linked with a large-scale shift of the North Atlantic thermohaline circulation (THC) to a near glacial mode with a consequent reduction in northward heat transport. This shift in the THC may have been triggered by a discharge of Laurentide ice, combined with meltwater inputs from several locations around the North Atlantic. Further study of the events leading up to the Younger Dryas is necessary for improving theoretical understanding of abrupt climatic change, and for evaluating GCM models which seek to simulate the response of the THC to freshwater forcing(Nicholas 2009). With predicted increases in freshwater input to the North Atlantic resulting from increases in atmospheric CO2, a future shift in the THC is a possibility. Predicting the magnitude and climatic consequences of such an event depends upon further study of the Younger Dryas and of other abrupt palaeoclimatic changes which involved the THC. The timing of the Younger Dryas was first established using radiocarbon dating and assigned to the approximate interval 11,000 to 10,000 years before the present (BP). We will write a custom essay sample on Younger Dryas in the UK specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Younger Dryas in the UK specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Younger Dryas in the UK specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The radiocarbon timescale is now known to be in error for the late-glacial period, owing to rapid changes in global carbon reservoirs at that time. The true date of the Younger Dryas has now been established using cores of ice drilled from the Greenland ice sheet. Careful study of ice layers that built up near the centre of the ice sheet has established that the onset of the Younger Dryas was about 12,800 calendar years ago, and its termination about many years ago. The Greenland ice cores provide such a clear and strong record of the Younger Dryas that they can be used as a yardstick against which other types of evidence can be compared(Lehman 1992). Reconstructed temperatures, based on the isotopic composition of oxygen locked up in the ice, are about 7 degrees C colder in central Greenland during the Younger Dryas than in the preceding and succeeding warm periods. The ice-core record shows that the cooling at the start of the Younger Dryas was gradual, but the warming at the end was exceedingly rapid, occurring within a few years or decades at most. The rapidity of this climatic change, which is also recorded in marine and terrestrial records from around the North Atlantic, is one of the most intriguing aspects of the Younger Dryas; it is also characteristic of other relatively short-lived episodes during the last glacial period. The Greenland ice cores also record changes in atmospheric composition. Air bubbles trapped within the ice show that during the Younger Dryas concentrations of the greenhouse gases, carbon dioxide and methane, were lower than modern natural levels, and were similar to those of full glacial times. Methane in particular shows a large oscillation, closely following the pattern of temperature change. Finally, precipitation changed during the Younger Dryas, with snow accumulation about half of that for the succeeding early Holocene(Broeker 1990). This evidence for low precipitation is compatible with the temperature record, since cold air cannot carry as much moisture as warm air. In Europe, the Younger Dryas cooling is most pronounced in Britain, southern Scandinavia, northern Germany, the Low Countries, and northern France, and declines in importance to the north, east, and south. On the other side of the Atlantic, the event is clearly recognizable in the maritime provinces of Canada and the north-eastern United States, but is less apparent further west. A short-lived cooling is also indicated by some pollen records from the Pacific coast of North America, as far north as Alaska. some scientists believe that the Younger Dryas can be recognized in pollen profiles from the northern Andes, Chile, Japan, and Australia, but others disagree. There is intriguing evidence that the Younger Dryas brought changes in wind circulation and moisture balance to some low-latitude continental areas(Bond 1993). Lake levels in East Africa fell, indicating dry conditions and a reduction in the on-land transfer of moist air from over the Indian Ocean. The Tibetan plateau is also thought to have been arid at that time. In contrast, the plateaux of Central China appear to have had a wetter climate than in the preceding and succeeding periods. This wet climate has been attributed to a heightened temperature gradient between a warm land surface and a relatively cool western Pacific, and a consequent strengthening of the summer monsoon. Additionally, the Altiplano in the high Andes also appears to have been wetter at that time. The climate of the Younger Dryas created the conditions for glacier expansion, especially in north-west Europe. The Scandinavian ice sheet, which had been in retreat until about 12-000 (radiocarbon) years ago, underwent a significant readvance and formed prominent moraines at the limit of ice advance in Norway, Sweden, and Finland. Ice cover in Britain had probably been reduced to isolated remnants in Scotland between 13-000 and 12-000 radiocarbon years ago, but during the Younger Dryas a substantial ice field formed over the western Scottish Highlands (locally known as the Loch Lomond readvance) and smaller mountain glaciers became established elsewhere in Britain and Ireland. Younger Dryas glacier advances of smaller magnitude have been identified in the European Alps, the North American Cordillera, the Andes, and New Zealand, although dating evidence is fragmentary and far from certain in many areas. In some areas, the glacial record hints at climatic changes within the Younger Dryas(Atkinson 1987). In western Scotland, retreat of some glaciers was well under way before the temperature increase that marked the end of the stade, suggesting that deglaciation may have begun in response to a decrease in snowfall. Changes in the abundance of certain pollen types also indicate that the Younger Dryas in north-west Europe consisted of a moist, cold period followed by a more arid (but still cold) period.